Stress Less Sundays
Weekly dispatches on regulation
Practical insights on Stress, Breathwork, Self-Regulation and nervous system science for better living. No noise. One email, every Sunday.
JONATHAN BARTLETT
Developer · 3G Breathing Protocol

x is a researcher, practitioner, and systems integrator working at the intersection of respiratory physiology and autonomic regulation. The 3G Protocol emerged from a question: "Why do practitioners report meaningful effects from nasal breathing at concentrations orders of magnitude below the clinical doses used in intensive care, and what does that gap actually mean?" The answer led to a formal theoretical framework, that reframes thirty years of inhaled nitric oxide dosing data. His practice is built on the same principle that drives the research: understanding the mechanism first, then designing the intervention around it. He works with individuals and organisations on stress physiology, nervous system regulation, and performance under pressure, guided by the principle that all choices stem from the physiological and emotional state they arise in.

VISIT APPLIED REGULATION →
WHAT IS THE 3G PROTOCOL?
Three-Gas Physiological Regulation
Research Protocol · Educational Use

3G stands for three gases nitric oxide, oxygen, and carbon dioxide the gases your body uses to regulate how efficiently you breathe and how well your nervous system recovers from stress.

Most breathing practices focus on one of these. The 3G Protocol works with all three at once, using a specific sequence that takes between seven to fifteen minutes.

WHAT HAPPENS IN A SESSION

You begin with a nasal inhale, followed by humming for ten seconds with your mouth closed. This vibrates your paranasal sinuses and floods your nasal cavity with a concentrated burst of nitric oxide, a signalling gas your body produces naturally that relaxes the small blood vessels in your lungs and improves how efficiently oxygen moves into your bloodstream. You then inhale immediately through your nose in a specific two-stage pattern designed to capture that burst before it is lost with your exhalation.

You then slow your breathing to around four to five breaths per minute, holding briefly after each inhale and exhale. The holds allow CO₂ to accumulate slightly, which triggers your blood to release oxygen more readily to your tissues, a well-established mechanism called the Bohr effect. At this pace, your breathing and heart rate begin to synchronise in a way associated with nervous system balance and recovery.

Two cycles of this takes approximately 7 minutes. Four cycles takes around 14 minutes.

WHO IS IT DESIGNED FOR?

The protocol is designed for people whose breathing patterns are working against them, habitual mouth-breathers, those under chronic stress, individuals recovering from illness, or anyone whose body is working harder than it should for the oxygen it needs. It's designed to help induce a fast state change to bring a greater state of clarity, peace and self-regulation. Healthy individuals can practise it too, though the physiological benefit is proportionally greater in those with room to improve.

THE SCIENCE

The 3G Protocol is a proposed mechanistic framework, not a clinically proven treatment. The full rationale, with 73 citations, is documented in a publicly archived protocol paper available on the Open Science Framework. The paper invites empirical investigation rather than claiming demonstrated efficacy.

READ THE FULL PROTOCOL PAPER →
AUDIO
3G Breathing Protocol · v3.0
Led by
0:00 --:--
OSF REPOSITORY
Research Protocol · Educational Use

3G BREATHING

THREE-GAS PHYSIOLOGICAL REGULATION

(NO • O₂ • CO₂)

Nasal protocol modulating nitric oxide, oxygen and CO₂ simultaneously to optimise delivery & stabilise the nervous system • OSF Archived v3.0

DISCLAIMER: This protocol is for educational purposes only. I am not a medical doctor. Consult a healthcare professional before beginning any respiratory intervention. Full documentation and dataset archived at the OSF Repository.

ORCID: 0009-0007-0991-8116
COPYRIGHT: © 2026 Jonathan Bartlett. The 3G Breathing Protocol is made available under a Creative Commons Attribution 4.0 International License (CC-BY 4.0). You are free to share, teach, adapt, and build upon this work for any purpose, including commercially, provided you credit Jonathan Bartlett as the originator, reference the protocol by name, and link to 3gbreathing.com.